Journal: Molecular medicine reports

Article Title: IL-22 exacerbates the severity of CVB3-induced acute viral myocarditis in IL-17A-deficient mice.

doi: 10.3892/mmr.2013.1323

Figure Lengend Snippet: Figure 2. Neutralization of IL-22 in IL-17A-/- mice promoted viral replication and decreased the production of TNF-α, IL-6 and IFN-γ. (A) The levels of cardiac CVB3 titers on day 14. Data show the mean values of CVB3 PFU/g of heart. (B) The relative expression levels of cardiac CVB3 RNA in different groups. (C-E) The result of statistical analysis for the alterations in cardiac TNF-α, IL-6 and IFN-γ mRNA, as measured by RT-PCR. (F) The correlation analysis of cardiac CVB3 RNA, cardiac IL-22 mRNA and IFN-γ mRNA. Each point represents an individual mouse. *P<0.01 and #P<0.05 versus the normal, AVMC and IgG groups. **P<0.01 and ##P<0.05 versus the AVMC, anti-IL-22 and IgG groups. Values are presented as the mean ± SD. IL, interleukin; CVB3, coxsackie virus B3; RT-PCR, real-time polymerase chain reaction; AVMC, acute viral myocarditis; SD, standard deviation.

Article Snippet: For in vivo IL-22 neutralization, a total of 32 IL-17A-/- mice were randomly divided into four groups: Mice in the AVMC group were injected with CVB3 and PBS (50 μg per mouse, n=8); in the anti-IL-22 Ab group, mice were administered with CVB3 and anti-IL-22 Ab (AF582; 50 μg per mouse; R&D Systems, Inc., Minneapolis, MN, USA; n=8); IgG control group, mice were injected with CVB3 and normal IgG control (AB-108-C; 50 μg per mouse; R&D Systems; n=8); and in the normal group, IL-17A-/- mice received no treatments (n=8).

Techniques: Neutralization, Expressing, Reverse Transcription Polymerase Chain Reaction, Virus, Real-time Polymerase Chain Reaction, Standard Deviation

Journal: Molecular medicine reports

Article Title: IL-22 exacerbates the severity of CVB3-induced acute viral myocarditis in IL-17A-deficient mice.

doi: 10.3892/mmr.2013.1323

Figure Lengend Snippet: Figure 3. Neutralization of IL-22 in IL-17A-/- mice decreased the levels of IL-22 and STAT3. (A) Results of the statistical analysis for the alteration of serum IL-22 protein levels, as investigated by ELISA. (B) The relative cardiac expression levels of IL-22, as analyzed by RT-PCR. (C) The correlation analysis of cardiac CVB3 RNA and cardiac IL-22 mRNA. (D and E) Results of the statistical analysis for the levels of cardiac STAT3 mRNA and STAT3 proteins, as mea sured by RT-PCR and western blot analysis, respectively. (F) Representative images for the levels of cardiac STAT3 proteins from each treated group. #P<0.05 versus the normal, AVMC and IgG groups. *P<0.01 versus the AVMC, anti-IL-22 and IgG groups. Values are presented as the mean ± SD. IL, interleukin; STAT3, activator of transcription 3; RT-PCR, real-time polymerase chain reaction; CVB3, coxsackievirus B3; SD, standard deviation; GAPDH, glyceralde hyde phosphate dehydrogenase.

Article Snippet: For in vivo IL-22 neutralization, a total of 32 IL-17A-/- mice were randomly divided into four groups: Mice in the AVMC group were injected with CVB3 and PBS (50 μg per mouse, n=8); in the anti-IL-22 Ab group, mice were administered with CVB3 and anti-IL-22 Ab (AF582; 50 μg per mouse; R&D Systems, Inc., Minneapolis, MN, USA; n=8); IgG control group, mice were injected with CVB3 and normal IgG control (AB-108-C; 50 μg per mouse; R&D Systems; n=8); and in the normal group, IL-17A-/- mice received no treatments (n=8).

Techniques: Neutralization, Enzyme-linked Immunosorbent Assay, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot, Real-time Polymerase Chain Reaction, Standard Deviation

Journal: Acta Neuropathologica Communications

Article Title: Increased mitochondrial activity in a novel IDH1-R132H mutant human oligodendroglioma xenograft model: in situ detection of 2-HG and α-KG

doi: 10.1186/2051-5960-1-18

Figure Lengend Snippet: Phenotypic characteristics of the IDH1 mutant xenograft model. Low ( A ) and high ( B ) magnifications of H&E-stained sections of E478 xenografts in mouse brain, showing diffuse infiltration throughout both hemispheres (note that infiltrative strands of cancer cells are interspersed in white matter [B]). C ) Ki-67 staining resulted in a proliferation index of approximately 34%. D ) Immunohistochemical anti-CD34 staining shows abundant presence of florid microvascular proliferations. E ) Immunostaining of mouse IgG shows limited and focal leakage of IgG from the tumor vasculature. The arrowhead in the low-magnification inset indicates the area depicted. F ) Blood vessels in the tumor express GLUT-1 that is characteristic for endothelial cells forming the blood–brain barrier. Cancer cells do not express GLUT-1, indicating that the tumor is not hypoxic. Size bars in A: 1 mm, B-F 100 μm.

Article Snippet: H&E and immunohistochemical stainings were performed as described previously [ ] including the use of antibodies directed against IDH1-R132H (clone H09, Dianova, Hamburg, Germany), Ki67 (for proliferation index assessment, clone Sp6, Neomarkers, Fremont, CA), cleaved caspase 3A (for detection of apoptotic cells, clone C92-605, BD Pharmingen, Franklin Lakes, NJ), CD34 (for endothelial cell staining, clone MEC14.7, Hycult Biotech, Uden, The Netherlands), GLUT-1 (Neomarkers), mouse IgG (Vector, Burlingame, CA), MCT-1 and MCT-4 (clones C-20 and H-90 respectively, Santa Cruz, CA).

Techniques: Mutagenesis, Staining, Immunohistochemical staining, Immunostaining